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Codon Usage Pattern of Metallothionein Genes in the Poplar Genome

Author(s):

Junkai Zhi, Jian Zhang, Jian Li, Hao Zhang and Jichen Xu*   Pages 1 - 8 ( 8 )

Abstract:


Metallothioneins (MTs) are important proteins for phytoremediation, and are widely involved in heavy metal uptake, transport, and enrichment. Based on MT sequencecharacteristicss, 10 MT genes were identified in the Populus trichocarpa genome. These genes and were categorized into four types in the Populus trichocarpa genome of plant MTs based on their sequences. Their open reading frames of the poplar MT genes ranged from 201 bp to 288 bp in length. The theoretical isoelectric point of poplar MTs ranged from 4.4 to 7.99 with an average value of 5.28. The amino acid similarity among poplar MT sequences ranged from 10.14% to 98.67%. A codon usage frequency analysis revealed a codon usage bias presented in the 10 poplar MT genes, with 21 high-frequency codons detectedtotally. in the 10 poplar MT genes. These results reveal the characteristics of poplar MTs, and provide the basis for further studies on their functional mechanisms and applications in phytoremediation.

Keywords:

Populus trichocarpa; metallothionein; domain; high-frequency condon; cysteine

Affiliation:

National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083



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