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Bioinformatics Analysis of Quantitative PCR and Reverse Transcription PCR in Detecting HCV RNA

Author(s):

Wei Liu, Xiwen Jiang, Yue Liu and Qingsong Ma*   Pages 1 - 6 ( 6 )

Abstract:


Objective: This research aimed to make comparisons of sensitivity and specificity between quantitative real time polymerase chain reaction (Q-PCR) and reverse transcription PCR (RT-PCR) in detecting the ribonucleic acid (RNA) expression levels of hepatitis C virus (HCV). Methods: 121 patients suffering from hepatitis C and 98 healthy participants with normal liver functions were identified. The venous blood collections were carried out, were subjected to detect the expression levels of HCV RNA via Q-PCR and RT-PCR. And then, the data obtained from these above two detection methods were compared, including the sensitivity and specificity. Results: In terms of Q-PCR, the positive rate of HCV RNA was 72.16%, which was significantly higher when compared with 55.26% of RT-PCR. After statistical analysis, the difference between them was statistically significant (P<0.05). Among the healthy participants, 4 cases were false positive by means of RT-PCR, there was the possibility of missed diagnosis when the samples were evaluated by Q-PCR. Conclusion: The Q-PCR detection technology performed well in testing HCV, with pretty high sensitivity and specificity. Nevertheless, the false negative results obtained from Q-PCR could not be avoided. In clinical practice, these above two detection methods should be referred to, in order to avoid missed diagnosis.

Keywords:

Hepatitis C virus; Ribonucleic acid; quantitative real time polymerase chain reaction

Affiliation:

Luhe Hospital Capital Medical University, Beijing, DAAN Gene Co., Ltd. Of Sun Yat-sen University Guangzhou, DAAN Gene Co., Ltd. Of Sun Yat-sen University Guangzhou, Qian`an traditional Chinese medicine hospital, Qian



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