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Structural and Functional Annotation of Virulent Hypothetical Proteins in Chlamydia trachomatis: An In-Silico Approach

Author(s):

Muhammad Naveed*, Muhammad Zubair Mehboob, Aadil Hussain, Khadija Ikram, Attha Talat and Nadia Zeeshan  

Abstract:


Though after a start of genome sequencing most of the protein sequences are deposited in databases, some proteins remain to be annotated and functionally uncharacterized. Analyzing and annotating the hypothetical proteins of Chlamydia trachomatis L2C, which is a gram-negative pathogen bacterium involved in causing severe disorders like lymphogranuloma venereum, nongonococcal urethritis and cervicitis, can help to understand its pathogenicity and therapeutic hotspots. Its genome encodes total of 221 hypothetical proteins and out of these, 14 hypothetical proteins are declared as virulent by virulence prediction server (VirulentPred). In this study, functional and structural analysis was carried out by conserve domain finding servers, protein function annotators and physiochemical properties predictors. Protein-protein interactions studies revealed the involvement of these virulent HPs in number of pathways which would be of interest for drug designers. Only 8 out of 14 models were verified as stable 3D structures. Classifier tool was used to classify the virulent hypothetical proteins into enzymes, membrane protein, transporter and regulatory protein groups. Our study would help to understand mechanisms of pathogenesis and new potential therapeutic targets for a couple of diseases caused by C. trachomatis.

Keywords:

Nongonococcal urethritis, virulent hypothetical proteins;, elementary bodies;, Chlamydia trachomatis

Affiliation:

Department of Biotechnology, Faculty of life sciences, University of Central Punjab, Lahore, Pakistan; Department of Biochemistry and Biotechnology, University of Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat



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