Neha Srivastava, Shifa Ansari and Prachi Srivastava* Pages 1 - 8 ( 8 )
Background: Neurodevelopmental Disorders (NDDs) are impairment of the growth and development of the brain or central nervous system, which occurs at the developmental stage. This can include developmental brain dysfunction, which can manifest as neuropsychiatric problems or impaired motor function, learning, language or non-verbal communication. These include the array of disorder, including: Autism Spectrum Disorders (ASD), Attention Deficit Hyperactivity Disorders (ADHD) etc. There is no particular diagnosis and cure for NDDs. These disorders seem to result from a combination of genetic, biological, psychosocial and environmental risk factors. Diverse scientific literature reveals the adverse effect of environmental factors specifically, exposure of pesticides, which leads to growing number of human pathological conditions; among these, neurodevelopmental disorder is an emerging issue nowadays. Objective: The current study focused on insilico identification of potential drug targets for pesticides induced neurodevelopmental disorder including Attention Deficit Hyperactivity Disorder (ADHD) and Autism Spectrum Disorder (ASD) and to design potential drug molecule for the target through drug discovery approaches. Method: We identified 139 candidate genes for ADHD and 206 candidate genes for ASD from the NCBI database for detailed study. Protein-protein interaction network analysis was performed to identify key genes/proteins in the network by using STRING 10.0 database and Cytoscape 3.3.0 software. The 3D structure of target protein was built and validated. Molecular docking was performed against twenty seven possible phytochemicals i.e. beta amyrin, ajmaline, serpentine, urosolic, huperzine A etc. having neuroprotective activity. The best-docked compound was identified by the lowest Binding Energy (BE). Further, the prediction of drug-likeness and bioactivity analysis of leads were performed by using molinspiration cheminformatics software. Result & Conclusion: Based on betweenness centrality and node degree as a network topological parameter, solute carrier family 6 member 4 (SLC6A4) was identified as a common key protein in both the networks. 3-D structure of SLC6A4 protein was designed and validated respectively. Based on the lowest binding energy, beta amyrin (B.E = -8.54 kcal/mol) was selected as a potential drug candidate against SLC6A4 protein. Prediction of drug-likeness and bioactivity analysis of leads showed drug candidate as a potential inhibitor. Beta amyrin (CID: 73145) was obtained as the most potential therapeutic inhibitor for ASD & ADHD in human.
Autism spectrum disorders (ASD), Attention Deficit Hyperactivity Disorder (ADHD), STRING 10.0, Cytoscape 3.3.0, Molecular docking.
A.P.J. Abdul Kalam Technical University, Lucknow, UP, AMITY Institute of Biotechnology, AMITY University Uttar Pradesh Lucknow, UP2. AMITY Institute of Biotechnology, AMITY University Uttar Pradesh Lucknow, UP, AMITY Institute of Biotechnology, AMITY University Uttar Pradesh Lucknow, UP2. AMITY Institute of Biotechnology, AMITY University Uttar Pradesh Lucknow, UP