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Proteome Mining For The Identification Of Putative Drug Targets For Human Pathogen Clostridium Tetani

Author(s):

Anum Munir*, Shaukat Iqbal Malik* and Khalid Akhtar Malik  

Abstract:


Background: Clostridium tetani are rod-like, anaerobic types of pathogenic bacteria of the genus Clostridium. It is Gram-positive in nature and appears as a tennis racket or drumsticks on staining with the dye. Methods: subtractive proteome analysis of C. tetani is performed. The proteins are subjected to blast analysis against Homo sapiens to exclude homologous proteins. The Essential Genes database is used to determine the essential proteins of the pathogen. These essential proteins are further analyzed to predict the metabolic pathways in which they are involved. Results: The study has recognized 29 essential genes and 20 unique pathways of 2314 proteins as potential drug targets. Out of 29 proteins, 3 membrane proteins are also identified as putative drug targets. Conclusion: Virtual screening in contrast to these target proteins can be beneficial in the detection of novel clinical compounds for the C. tetani infections in Homo sapiens.

Keywords:

drug targets, essential genes, membrane proteins, metabolic pathways, putative

Affiliation:

Department of Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad, Department of Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad , Department of Mechanical Engineering (SMME), National University of Science and Technology, Islamabad



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