Sarah Rizwan Qazi, Noor ul Haq, Shakeel Ahmad and Samina N. Shakeel* Pages 196 - 203 ( 8 )
Background: Previous methods used to discover cis-regulatory motifs in promoter region of plant genes possess very limited performance, especially for analysis of novel and rare motifs. Different plant genes have differential expression under different environmental or experimental conditions and modular regulation of cis-regulatory sequences in promoter regions of the same or different genes. It has previously been revealed that Heat Shock Proteins (HSPs) creation is correlated with plant tolerance under heat and other stress conditions. Regulation of these HSP genes is controlled by interactions between heat shock factors (HSFs) with cis-acting motifs present in the promoter region of the genes. Differential expression of these HSP genes is because of their unique promoter architecture, cis-acting sequences and their interaction with HSFs.
Objective: A versatile promoter analysis tool was proposed for identification and analysis of promoters of HSPs.
Methods: Heat Shock Element Analysis Tool (HSEAT) has been implemented in java programming language using pattern recognition approach. This tool has build-in MS access database for storing different motifs.
Results: HSEAT has been designed to detect different types of Heat Shock Elements (HSEs) in promoter regions of plant HSPs with integration of complete analysis of plant promoters to the tool. HSEAT is user-friendly, interactive application to discover various types of HSEs e.g. TTC Rich Types, Gap Types and Prefect HSE as well as STRE in HSPs. Here we examined and evaluated some known HSP promoters from different plants using this tool with already available tools.
Conclusion: HSEAT has extensive potential to explore conserved or semi-conserved motifs or potential binding sites of different transcription factors for other stress regulating genes. This tool can be found at https://sourceforge.net/projects/heast/.
Heat shock element, heat shock proteins, plants, stress, motifs, transcription.
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University 45320, Islamabad, Department of Computer Science and Bioinformatics, Khushal Khan Khattak University 27200, Karak, Khyber-Pakhtunkhwa, Pakistan Institute of Science and Technology (PINSTECH), Nilore, Islamabad, Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University 45320, Islamabad