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Genetic Diversity of Blattella germanica Isolates from Central China based on Mitochondrial Genes

[ Vol. 14 , Issue. 7 ]

Author(s):

Pan Wei, XiaoDong Xie, Ran Wang, JianFeng Zhang, Feng Li, ZhaoPeng Luo, Zhong Wang, MingZhu Wu, Jun Yang and PeiJian Cao*   Pages 574 - 580 ( 7 )

Abstract:


Background: Blattella germanica is a widespread urban invader insect that can spread numerous types of human pathogens, including bacteria, fungi, and protozoa. Despite the medical significance of B. germanica, the genetic diversity of this species has not been investigated across its wide geographical distribution in China.

Objective: In this study, the genetic variation of B. germanica was evaluated in central China.

Methods: Fragments of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the 16S rRNA gene were amplified in 36 B. germanica isolates from 7 regions. The sequence data for COI and 16S rRNA genes were analyzed using bioinformatics methods.

Results: In total, 13 haplotypes were found among the concatenated sequences. Each sampled population, and the total population, had high haplotype diversity (Hd) that was accompanied by low nucleotide diversity (Pi). Molecular genetic variation analysis indicated that 84.33% of the genetic variation derived from intra-region sequences. Phylogenetic analysis indicated that the B. germanica isolates from central China should be classified as a single population. Demographic analysis rejected the hypothesis of sudden population expansion of the B. germanica population.

Conclusion: The 36 isolates of B. germanica sampled in this study had high genetic variation and belonged to the same species. They should be classified as a single population. The mismatch distribution analysis and BSP analysis did not support a demographic population expansion of the B. germanica population, which provided useful knowledge for monitoring changes in parasite populations for future control strategies.

Keywords:

Blattella germanica, COI, 16S rRNA, genetic variation, phylogeny, mitochondrial.

Affiliation:

China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001

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