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Analysis of Germin-Like Protein Genes (OsGLPs) Family in Rice Using Various In Silico Approaches

Author(s):

Muhammad Ilyas*, Muhammad Irfan, Tariq Mahmood, Hazrat Hussain, Latif Ur Rahman and Ijaz Naeem   Pages 1 - 17 ( 17 )

Abstract:


Background: Germin-like proteins (GLPs) play an important role against various stresses. Rice contains 43 GLPs, among which many remain functionally unexplored. The computational analysis will provide significant insight into their function.

Methods: Physiochemical properties, sub-cellular localization, domain composition, N-glycosylation and Phosphorylation sites, 3D structural models of the OsGLPs were predicted using various bioinformatics tools. Functional analysis was carried out with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Blast2GO servers. Expression profile of the OsGLPs was predicted by retrieving the data for expression values from tissue-specific and hormonal stressed array libraries of RiceXPro. Their phylogenetic relationship was computed using Molecular and evolutionary genetic analysis (MEGA6) tool.

Results: Most of the OsGLPs are stable in a cellular environment with prominent expression in the extracellular region (57%) and plasma membrane (33%). Besides, 3 basic cupin domains, 7 more were reported, among which NTTNKVGSNVTLINV, FLLAALLALASWQAI, and MASSSF were common to 99% of the sequences, related to bacterial pathogenicity, peroxidase activity, and peptide signal activity respectively. Structurally, OsGLPs are similar but functionally they are diverse with novel enzymatic activities of oxalate decarboxylase, lyase, peroxidase, and oxidoreductase. Expression analysis revealed prominent activities in root, endosperm, and leaves. OsGLPs were strongly expressed by abscisic acid, auxin, gibberellin, cytokinin, and brassinosteroid. Phylogenetically they showed polyphyletic origin with a narrow genetic background of 0.05%. OsGLPs of chromosome 3, 8, 12 are functionally more important due to their defensive role against various stresses through co-expression strategy.

Conclusion: The analysis will help to utilize OsGLPs in future food programs.

Keywords:

OsGLPs, Phylogenetic, rice, expression, In silico , Germin-like proteins

Affiliation:

Department of Botany, University of Swabi, Swabi-23561, Khyber Pakhtunkhwa, Department of Botany, University of Swabi, Swabi-23561, Khyber Pakhtunkhwa, Department of Botany, Faculty of Biological Sciences, Quaid-I -Azam University, Islamabad 45320, Department of Biotechnology, University of Swabi, Swabi-23561, Khyber Pakhtunkhwa, Department of Biotechnology, University of Swabi, Swabi-23561, Khyber Pakhtunkhwa, Department of Biotechnology, University of Swabi, Swabi-23561, Khyber Pakhtunkhwa



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