Behnam Bakhshi* and Ehsan Mohseni Fard Pages 455 - 462 ( 8 )
Background: Aegilops tauschii is a wild relative of bread wheat. This species has been reported as the donor of bread wheat D genome. There are also several reports that mentioned the importance of Ae. tauschii in biotic and abiotic stress tolerance. On the other hands, miRNAs have been reported as the essential regulatory elements in stress response.Objective: Therefore, it is important to discover novel miRNAs involved in stress tolerance in this species. The aim of the current study was to predict novel miRNAs in Ae. tauschii and also uncover their potential role in stress response. Method: For this purpose, ESTs, TSAs, and miRBase databases were obtained and used to predict new miRNAs. Results: Our results discovered nine novel stem-loop miRNAs. These predicted miRNAs could be introduced as the new members of previously identified miRNA families in Ae. tauschii, including miR156, miR168, miR169, and miR319. The result indicating that miR397 and miR530 are novel families in this species. Furthermore, several novel stem-loop miRNAs predicted for T. aestivum showed remarkable similarities to novel Ae. tauschii stem-loops. Conclusion: Our results demonstrated that predicted novel miRNAs could play a significant role in stress response.
Ae. tauschii, wheat, microRNAs, EST, TSA.
Horticulture Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan