Pin Lyu*, Jianhua Hou, Haifeng Yu and Huimin Shi
Background: Sunflower (Helianthus annuus L.) is an important oil crop only after soybean, canola and peanuts. A high quality genetic map is the foundation of marker-assisted selection (MAS). But for this species, the high-density maps were reported limitedly.
Objective: In this study, we proposed to construct a high-density genetic linkage map by an F7 population of sunflower using SNP and SSR Markers.
Method: The SLAF-seq strategy was employed to further develop SNP markers with SSR markers to construct the high-density genetic map by the HighMap software.
Results: A total of 1,138 million paired-end reads (226Gb) were obtained and 518,900 SLAFs were detected. Of the polymorphic SLAFs, 2,472,245 SNPs were developed and finally 5,700 SNPs are ideal to construct genetic map after filtering. The final high-density genetic map included 4,912 SNP and 93 SSR markers distributed in 17 linkage groups (LGs) and covered 2,425.05 cM with an average marker interval of 0.49 cM.
Conclusion: The final result demonstrated that the SLAF-seq strategy is suitable for SNP markers detection. The map in this report considered to be one of the most high-density genetic linkage map in sunflower and could lay a foundation for quantitative trait loci (QTLs) fine mapping or map-base gene cloning.
sunflower (Helianthus annuus L.), genetic linkage map, high-density, SLAF-seq, SNP, SSR
College of Agriculture, Inner Mongolia Agricultural University, College of Agriculture, Inner Mongolia Agricultural University , Crop Research Institute, Inner Mongolia Academy of Agricultural and Husbandry Sciences, College of Agriculture, Inner Mongolia Agricultural University