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NPalmitoylDeep-PseAAC: A Predictor of N-Palmitoylation sites in Proteins using Deep Representations of Proteins and PseAAC via Modified 5-steps Rule

Author(s):

Sheraz Naseer*, Waqar Hussain, Yaser Daanial Khan and Nouman Rasool   Pages 1 - 12 ( 12 )

Abstract:


Background: Among all the major Post-translational modification, lipid modifications possess special significance due to their widespread functional importance in eukaryotic cells. There exist multiple types of lipid modifications and Palmitoylation, among them, is one of the broader types of modification, having three different types. The N-Palmitoylation is carried out by attachment of palmitic acid to an N-terminal cysteine. Due to the association of N-Palmitoylation with various biological functions and diseases such as Alzheimer’s and other neurodegenerative diseases, carrying out important processes in the life cycle of various pathogens, its identification is very important.

Objective: The in vitro, ex vivo and in vivo identification of Palmitoylation is laborious, time-taking and costly. There is a dire need of an efficient and accurate computational model to help researchers and biologists identifying these sites, in an easy manner. Herein, we propose a novel prediction model for identification of N-Palmitoylation sites in proteins.

Method: Proposed prediction model is developed by combining the Chou’s Pseudo Amino Acid Composition (PseAAC) with deep neural networks. We used well-known deep neural networks (DNNs) for both the tasks of learning a feature representation of peptide sequences and developing prediction model to perform classification.

Results: Among different DNNs, Gated Recurrent Unit (GRU) based RNN model showed highest scores in terms of accuracy, and all other computed measures, and outperforms all the previously reported predictors.

Conclusion: The proposed GRU based RNN model can help identifying N-Palmitoylation in a very efficient and accurate manner which can help scientists understand the mechanism of this modification in proteins.

Keywords:

N-Palmitoylation, DNNs, Deep features, 5-steps rule, PseAAC

Affiliation:

Department of Computer Science, School of Systems and Technology, University of Management and Technology, P.O. Box 10033, C-II, Johar Town, Lahore 54770, National Center of Artificial Intelligence, Punjab University College of Information Technology, University of the Punjab, Lahore, Department of Computer Science, School of Systems and Technology, University of Management and Technology, P.O. Box 10033, C-II, Johar Town, Lahore 54770, Dr Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270



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