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MGB Block ARMS Real-Time PCR for Diagnosis of CYP2C19 Mutation in a Chinese Population

[ Vol. 14 , Issue. 5 ]

Author(s):

Xi-Wen Jiang*, Yue Liu, Tao-Sheng Huang and Xiao-Ya Zhu   Pages 391 - 399 ( 9 )

Abstract:


Background: CYP2C19 is an important genetic factor modulating clopidogrel dose requirement.

Objective: Therefore, a simple and economic genotyping method for predicting the clopidogrel dose of patients would be useful in clinical applications.

Methods: In this study, the MGB blocker ARMS real-time PCR contained two forward primers, two MGB blockers and a common reverse primer have been used for CYP2C19*2, *3 and *17 substitutions.

Results: Results showed that heterozygotes and homozygotes of CYP2C19*2, *3 and *17 could be distinguished by the MGB blocker ARMS real-time PCR successfully. In the Chinese population, patients with allele frequencies of CYP2C19*2, *3, and *17 are 18.43%, 3.03% and 0.76%, respectively.

Conclusion: This study indicates that the MGB blocker ARMS real-time PCR will be a simple, economical method for the rapid detection of SNPs in CYP2C19.

Keywords:

MGB, blocker, ARMS, CYP2C19, genotyping, heterozygotes, homozygotes.

Affiliation:

DAAN Gene Co., Ltd. of Sun Yat-Sen University, 19 Xiangshan Road, Science Park, High & New Technology Development District, Guangzhou, Guangdong, DAAN Gene Co., Ltd. of Sun Yat-Sen University, 19 Xiangshan Road, Science Park, High & New Technology Development District, Guangzhou, Guangdong, DAAN Gene Co., Ltd. of Sun Yat-Sen University, 19 Xiangshan Road, Science Park, High & New Technology Development District, Guangzhou, Guangdong, DAAN Gene Co., Ltd. of Sun Yat-Sen University, 19 Xiangshan Road, Science Park, High & New Technology Development District, Guangzhou, Guangdong

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